Decolorization of Sudan Orange G by laccase purified from various Mushrooms

Abstract

Laccases are oxidoreductases that have great potential in various biotechnological processes due to
their high nonspecific oxidation capacity, the lack of a requirement for cofactors, and the use of readily
available oxygen as an electron acceptor. Laccases from fungi are employed in various
biotechnological processes mainly decolorization. In the present investigation, the fungusPleurotus
ostreatus, Ganoderma lucidum and Morchella esculenta of the Himalayan regionwere employed for
decolorization of Sudan Orange G. The study conducted showed that Pleurotus ostreatus and
Ganoderma lucidum were able to decolorize ~48% and ~36% Sudan Orange G respectively, in 28 days
at room temperature. Morchella esculenta was abletodecolorize~12 % in 28 days.
The extent of degradation for the dye by fungi can be evaluated with the help of UV-Visible
Spectrophotometer and High-Performance Liquid Chromatography.This paper describes the
development of a simple and sensitive chromatography method with reduced run time for the estimation
of biodegradation products of Sudan Orange G dye