SEPERATION AND SELECTION OF PGPRs FROM CHICKPEA RHIZOSPHERIC SOIL

Abstract

The present study aimed to isolate and screen native plant growth promoting bacteria from chickpea rhizosphere from Bhojia Institute of Life Sciences agriculture field. Three bacterial strains were isolated and purified on nutrient agar plates in vitro for the analysis of PGP characteristics. Among three isolates, bacterial isolate B and C were gram negative and morphologically rod shaped. Whereas strain A was Gram positive and rod shaped. All isolates were glucose fermenter. Strain B is partly positive for the fermentation of maltose, mannose, and fructose, positive for galactose and negative for xylulose and reffinose and cellulose. All strains were found to be catalase positive; TSI negative, negative for urease production and citrate test. Stains A and B were positive for triple sugar iron test and strain C was partly positive. Isolate A and B shown a positive result for indole production and phosphate solubilization. The results of this study provide a positive line for the use of these bacteria as plant growth promoting organism for seed inoculation studies.