In vivo Reproductive Toxicity evaluation of thiamethoxam using Drosophila melanogaster

  • Lovleen


Present research execution deals with reproductive toxicity evaluation of a systemic neonicotinoids insecticide, thiamethoxam, using Drosophila melanogaster, exposed to LC20 for 24 hours.  To achieve the specific target of standardization of exposure concentration, second instar larvae were exposed to serial dilution of thiamethoxam for 24 hours, subsequently, on the basic of observed mortality, exact value of LC20 was calculated by probit analysis. Afterward, adult flies were allowed to crossmate in two different sets; first set, include cross mating of treated male and normal female, whereas in second set treated female were allowed to cross mate with normal male. All set of experiments were repeated in the form of triplicate and control groups were also maintained simultaneously. Finally, fecundity of treated groups, for two different experimental sets, was compared with the natural population. It was observed a significant decrease in fecundity of treated flies as compare to control groups. Furthermore, it was scrutinized that thiamethoxam effect predominantly, when exposure is given to male only, as revealed from procure data. Subsequently, obtained results from different experimental test was analysed by Z-test, which indicated statistically significant result [p<0.05].