Comparative Analysis of Halophilic Bacteria isolates for PHB Production Utilizing Substrate and Media Modification Methods

  • Madhuri Girdhar


Production of PHB (poly-?-hydroxy-butyrate) from bacterial isolates is an interesting area of naturally occurring polymeric compound research. Current work investigates the isolation, characterization and production of PHB from halophilic bacteria of soil and water samples collected at Sambhar Lake in Rajasthan (India). Sudan black B stain was used to screen the bacterial strains for PHB. The extraction of PHB from PHB producing positive isolates (S1, S2, S3, S4 and W) was performed by chloroform and sodium hypochlorite method. Thin Layer chromatography (TLC) and FTIR were performed for qualitative analysis and measuring the purity of extracted PHB. The optimization strategies were utilized for the selection of isolates to study the PHB production. Further investigations were carried out for comparison of parameters of optimization viz., cheaper carbon sources, differential pH, modified specific media (MSM), differential specific media (DSM), salt tolerance capacity of isolates and their characterization with various biochemical tests. The maximum level of bacterial growth were obtained by DSM and MSM medium, pH levels 7.4 and 8, Sucrose, Starch and Lactose based carbon source at 1.5 %, along with 4M NaCl concentration.